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KMID : 0352419930120010043
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Keimyung Medical Journal
1993 Volume.12 No. 1 p.43 ~ p.54
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Effect of Parenteral Administration of Endotoxin on Changes of Serum and Hepatic Glutathione S-Transferase, Glutathione Peroxidase and Glutathione Reductase Activities in Rats
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Abstract
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The activities of the cytosolic, microsomal and mitochondrial glutatione S-tranferase (GST) which are detoxifying enzymes in thd liver were measured in order to evaluate the detoxifying ability of the liver to endotoxin. The activities of glutathione peroxidase(GSH-Px), glutathione reductase(GR) in the liver, GST and GR in the serum were also measured.
For administration of endotoxin, a dose of 5㎎ of endotoxin (lipopolysaccharide E, coli O26: B6, from Sigma chemical company, USA) per kg of body weight was administered through a right external jugular vein. Then the rats were killed after 3, 8 and 24 hours of injection with endotoxin to measure the activities of the above enzymes in serum and their liver.
The activities of the cytosokic and mocrosomal GST in the liver showed a significant decrease between 8 and 24 hours after endotoxin administration, but that of the motochondria showed no signifcant changes throughout the experiment. The activity of the GR in the liver showed a significant decrease at 24 hours after endotoxin adiministration. The activity of the GSH-Px in the liver showed a significant decrease at 8 hours after endotoxin administration. Serum GST and GR activitites showed a signifcant increase between 8 and 24 hours and between 3 and 24 hours after endotoxin administration respectively.
According to the results, cytosolic GST and hepatic GR leak into the blood through the damaged membrane of hepatocyte. And it is suggested that the synthesis of the microsomal GST in the liver is decreased.
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